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Ref #341 : Regulation of hepatocyte glutathione by amino acid precursors and cAMP in protein-energy malnourished rats
Author(s):
Goss PM, Bray TM, Nagy LE.
Date:
1994
Reference Type:
Journal
Periodical:
Journal of Nutrition
Issue:
124
Volume:
3
Page range:
323 - 330
Keywords:
Amino-acids, C-amp, Cells, Cysteine, Diet, Glutathione, Hormonal-control, In-vitro, Liver-cells, Losses, Malnutrition, Methionine, Precursors, Protein, Protein energy malnutrition, Protein-intake, Rats, Regulation, Synthesis, Treatment.
Abstract / Notes:
Hepatocytes isolated from male weanling Wistar rats fed on a very low protein diet (0.5 g/100 g diet) or on a diet adequate in protein for 2 weeks were used to investigate whether a loss of hormonal control could contribute to abnormal regulation of hepatic glutathione. Glutathione concentration in hepatocytes isolated from protein-energy malnourished rats was 82% lower than in controls. In vitro supplementation of isolated hepatocytes with oxothiazolidine-4-carboxylate or methionine increased glutathione concentration in hepatocytes from malnourished rats to concentrations equivalent to control cells. However, when hepatocytes were incubated with cysteine, total glutathione in malnourished rats exceeded that of controls. Treatment of cells from control rats with glucagon 50 nmol/litre or db-cAMP 1 mmol/litre decreased glutathione concentration by 25-43%. In contrast, the glutathione concentration in hepatocytes of rats fed on the low protein diet did not respond to treatment with glucagon or db-cAMP. Data indicate that glutathione synthesis is non-sensitive to regulation by cAMP in rats with protein-energy malnutrition. This loss of responsiveness to hormonal control may contribute to the abnormal homeostatic regulation of hepatic glutathione observed in protein-energy malnutrition342